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聯(lián)系人：蔣經(jīng)理
電話：4008750250
號(hào)碼：
手機(jī)：18066071954
地址：南京市棲霞區(qū)緯地路9號(hào)
Email： zhangxiangwen@cobioer.com

名稱	c-Kit N822K/BaF3
型號(hào)	CBP73279
報(bào)價(jià)
特點(diǎn)	c-Kit N822K/BaF3,母細(xì)胞：BaF3 KIT N822K 位于 Kit 蛋白（PMID：24205792）的蛋白激酶結(jié)構(gòu)域中的激活環(huán)內(nèi)。 N822K 導(dǎo)致 Kit 的組成型激活，增加 Erk1/2 和 Stat3 磷酸化，在培養(yǎng)中轉(zhuǎn)化，，導(dǎo)致 Kit 錯(cuò)誤定位到內(nèi)溶酶體，并已被確定為次要與伊馬替尼耐藥相關(guān)的突變。

CBP73279
I. Introduction
Cell Line Name：	c-Kit N822K/BaF3
Host Cell:	Ba/F3
Stability:	16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:	90% FBS+10% DMSO
Complete Culture Medium:	RPMI-1640+10%FBS+2ug/ml puromycin
Mycoplasma Status:	Negative

II.Background
KIT N822K lies within the activation loop in the protein kinase domain of the Kit protein (PMID: 24205792). N822K results in constitutive activation of Kit, increased Erk1/2 and Stat3 phosphorylation, is transforming in culture (PMID: 24205792, PMID: 31484543), leads to mislocalization of Kit to endolysosomes (PMID: 31484543), and has been identified as a secondary mutation associated with imatinib-resistance (PMID: 18488160).

III. Representative Data
1.WB of c-Kit N822K/BaF3
2. Sanger of c-Kit N822K/BaF3
3. Anti-proliferation assay Figure 3. CTG Proliferation Assay of BaF3 c-KIT N822K(C4).